Association between SNPs and Loss of Methylation Site on the CpG island of the Promoter Region of the Smoothened Gene, Potential Molecular Markers for Susceptibility to the Development of Basal Cell Carcinoma in the Brazilian Population

Document Type: Research Articles

Authors

1 Laboratory of Structural Molecular Biology and Oncogenetics - LBMEO, Federal University of Paraiba, João Pessoa - PB, Brazil.

2 Postgraduate Program in Cellular and Molecular Biology, Federal University of Paraiba, João Pessoa - PB, Brazil.

3 Department of Dermatology, Dermatological Clinic Santa Catarina, João Pessoa - PB - Brazil.

4 Postgraduate Program in Health Sciences; Santa Casa School of Medical Sciences of São Paulo; Sao Paulo – SP, Brazil.

5 Depathology of Pathology, Laboratory of Medical Analysis - UNILAB, João Pessoa - PB, Brazil.

6 6Pharmacy Department; State University of Paraíba; Campina Grande – PB, Brazil.

7 Postgraduate Program in Natural Sciences and Biotechnology, Federal University of Campina Grande - UFCG, Campus de Cuité – PB, Brazil.

8 Institute of Biological Sciences; Federal University of Pará; Belém - PA - Brazil Brazil.

9 Molecular Biology Department; Federal University of Paraiba; João Pessoa - PB, Brazil.

Abstract

Objective: Perform genotyping of SNPs in the promoter region of the SMO gene in BCC samples from patients from northeastern Brazil, and to determine if there is an association of these SNPs of the gene in question with the susceptibility to the development of the BCC. Methods: 100 samples of paraffined tissue from patients with histopathological diagnosis of BCC and 100 control samples were analyzed for each polymorphism by a newly developed genotyping method, the Dideoxy Single Allele Specific – PCR. The software Bioestat - version 5.3 and Haploview 4.2 were used for the statistical analysis. For all tests a P-value  

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