Role of Frozen Sections in the Evaluation of Moderate to SevereDysplasia during Uterine Cervix Conization


Background: The utility of frozen section examination (FSE) of cone specimens in evaluation of the resectionmargin status and in ruling out invasion in patients with high grade cervical intraepithelial neoplasia requiresevaluation.
Methods: Twenty patients with high grade cervical intraepithelial neoplasia who underwent conizationbiopsy and frozen section examination were studied in a prospective trial from March 2008 through September2009. The results with permanent paraffin sections were compared with those of FSE.
Results: Among the twentycases, 15 (75%) had the same results in frozen and permanent sections of cone biopsy specimens. Among theother 5 patients, 2 had high grade cervical intraepithelial neoplasia in frozen sections and 2 showed a lower gradewhile only one case was found in which the FSE result was CIN3 while the permanent section showed invasivecarcinoma which was of clinical importance and considered as significant. Paired sample t-testing showed nosignificant difference in the results of the two groups of frozen and permanent sections (P=0.716, CI=95%).
Conclusion: Frozen section evaluation of cervical cone biopsy specimens in patients with a diagnosis of CIN 3 isaccurate, efficient and cost-effective. Because of the great importance of missing even one case, further researchis highly recommended on this controversial subject.