Cytotoxic activity of artemisinin and derivatives in the presence and absence of holo-transferrin andexpression of genes involved in resistance of cancer cells were investigated in human cholangiocarcinoma (CL-6)and hepatocarcinoma (Hep-G2) cell lines in vitro. After incubation with the test drugs and 5-fluorouracil (5-FU) cytotoxicity was asessed by MTT assay. RNA was extracted after 24 hour exposure to holo-transferrin forinvesstigation of the expression of transferrin receptor 1 (TDR1), multidrug resistance 1 (MDR1), multidrugresistance protein 1 (MRP1), multidrug resistance protein 2 (MRP2), and multidrug resistance protein 3 (MRP3).The median IC50 of artemisinin, artesunate, β-artemeter, dihydroartemisinin and 5-FU were as follows: CL-6:339, 131, 354, 75, and 377 μM, respectively; Hep-G2: 268, 50, 233, 29, and 1,380 μM. Exposure to holo-transferrinhad no influence on sensitivity of either cell line to artemisinin derivatives, but resulted in a 3-fold increase inthe expression of TR1 and MDR1, and a 2-fold increase in the expression of MRP1 and MRP2 in CL-6 cells.With Hep-G2, a 3-fold increase in the expression of MDR1 and MRP3 and a 2-fold increase in expression ofMRP2 were observed. Dihydroartemisinin exhibited the most potent cytotoxic activity against both cell linesand holo-transferrin caused different patterns of expression of resistance-associated genes.
(2011). Cytotoxic Activity of Artemisinin Derivatives Against Cholangiocarcinoma (CL-6) and Hepatocarcinoma (Hep-G2) Cell Lines. Asian Pacific Journal of Cancer Prevention, 12(1), 55-59.
MLA
. "Cytotoxic Activity of Artemisinin Derivatives Against Cholangiocarcinoma (CL-6) and Hepatocarcinoma (Hep-G2) Cell Lines", Asian Pacific Journal of Cancer Prevention, 12, 1, 2011, 55-59.
HARVARD
(2011). 'Cytotoxic Activity of Artemisinin Derivatives Against Cholangiocarcinoma (CL-6) and Hepatocarcinoma (Hep-G2) Cell Lines', Asian Pacific Journal of Cancer Prevention, 12(1), pp. 55-59.
CHICAGO
, "Cytotoxic Activity of Artemisinin Derivatives Against Cholangiocarcinoma (CL-6) and Hepatocarcinoma (Hep-G2) Cell Lines," Asian Pacific Journal of Cancer Prevention, 12 1 (2011): 55-59,
VANCOUVER
Cytotoxic Activity of Artemisinin Derivatives Against Cholangiocarcinoma (CL-6) and Hepatocarcinoma (Hep-G2) Cell Lines. Asian Pacific Journal of Cancer Prevention, 2011; 12(1): 55-59.