Beclin1 Overexpression Inhibitis Proliferation, Invasion and Migration of CaSki Cervical Cancer Cells


The influence of the autophagy-related gene Beclin1 on proliferation, invasion and metastasis of the cervicalcancer CaSki cells and its possible mechanism in vitro were here targeted. After the overexpression vectorpcDNA3.1-Beclin1 and RNA interference vector pSUPER-Beclin1 were transfected into CaSki cells in vitro, stableexpression cell lines demonstration Beclin1 expression was upregulated, and VEGF and MMP-9 expression weredecreased, leading to cell arrest in the G0/G1 phase of the cell cycle. MTT assays further revealed proliferationof cells was significantly inhibited in Beclin1-overexpressing transfectant cells, with invasion and metastasisalso being inhibited in Transwell chamber assays. The present results suggest that Beclin1 inhibits invasionand metastasis of cervical cancer CaSki cells in vitro. Mechanisms probably involve Beclin1 inhibition of cellproliferation, and decreased expression of VEGF and MMP-9 proteins.