In order to verify effects of Yiqi Chutan Tang on lung cancer and assess molecular mechanisms involvedwe focused on size, tumor weight and the numbers of lung metastases and differential expression proteinspot information acquired by two-way fluorescence with a tumor difference gel electrophoresis (2D-DIGE)system, and differentially expressed proteins were identified by matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF-TOF). Differences were finally verified by Western blot andfluorescence quantitative PCR.We found that tumor size, tumor weight in Yiqi Chutan Tang treatment groupwere significantly less than that in model group (p<0.01), with a tumor growth inhibition rate of 57.2%. For geldiagram analysis of 2D-DIGE system, compared with model group, there were 44 expressed differentially proteinspots, of which 6 were up-regulated and 38 were down-regulated. Among these proteins, 37 (30 down-regulatedand 7 up-regulated) were successfully identified by MALDI-TOF-TOF. In conclusion, Yiqi Chutan Tang effectson LEWIS lung cancer appeared highly related to down-regulated expression of Hspd1, prolyl 4-hydroxylase,protein disulfide-isomerase A3 precursor, EG433182, heat shock protein 5 precursor, heat shock protein 9 andstress-induced phosphoprotein 1.
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