Effect of Luteolin on the Levels of Glycoproteins During Azoxymethane-induced Colon Carcinogenesis in Mice


Luteolin (LUT), a bioflavonoid has been used as a chemopreventive agent world-wide against chemicallyinduced cancer. Hence we designed an experiment to assess chemopreventive action of LUT on lipid peroxidation(LPO) and glycoconjugates in azoxymethane (AOM)-induced colon carcinogenesis. Colon cancer was induced by15 mg/body kg. body weight of AOM and administration of LUT (at the dose of 1.2 mg/kg. body weight) was tillend of the study. Analysis of lipid peroxidative end products such as protein carbonyl (PC), malonadehyde (MDA)and conjucated dienes (CD) demonstrated significant increase in in AOM-induced animals with reduction by LUT(p<0.05) . Increased levels of glycoconjugates such as hexose, hexosamine, sialic acid, fucose and mucoproteinwere analyzed in serum and colon tissues examined histopathologically by periodic acid Schiff’s (PAS) stainingwere also reversed by LUT l(p<0.05) . The secondary marker of colon cancer mucin depleted foci (MDF) wasassessed in control and experimental group of animals. A characteristic increase of MDF was observed in AOMinducedcolon cancer animals. Treatment with LUT decreased the incidence of MDF. These results suggest thatLUT alters the expression of glycoconjugates and suppress colon cancer. Hence, we speculate that LUT can beused as a chemopreventive agent to treat colon cancer.