Effect of Tissue Factor on Invasion Inhibition and Apoptosis Inducing Effect of Oxaliplatin in Human Gastric Cancer Cell


Objective: Tissue factor (TF) is expressed abnormally in certain types of tumor cells, closely related to invasionand metastasis. The aim of this study was to construct a human gastric cancer cell line SGC7901 stably-transfectedwith human TF, and observe effects on oxaliplatin-dependent inhibition of invasion and the apoptosis induction.
Methods: The target gene TF was obtained from human placenta by nested PCR and introduced into the humangastric cell line SGC7901 through transfection mediated by lipofectamine. Stably-transfected cells were screenedusing G418. Examples successfully transfected with TF-pcDNA3 recombinant (experimental group), and emptyvector pcDNA3 (control group) were incubated with oxaliplatin. Transwell chambers were used to show changein invasive ability. Caspase-3 activity was detected using a colorimetric method and annexin-V/PI doublestainingwas applied to detect apoptosis.
Results: We generated the human gastric cancer cell line SGC7901/TFsuccessfully, expressing TF stably and efficiently. Compared with the control group, invasion increased, whereascaspase-3 activity and apoptosis rate were decreased in the experimental group.
Conclusion: TF can enhancethe invasive capacity of gastric cancer cells in vitro. Its increased expression may reduce invasion inhibition andapoptosis-inducing effects of oxaliplatin and therefore may warrant targeting for improved chemotherapy.