Safety Assessment of Ovarian Cryopreservation and Transplantation in Nude Mice Bearing Human Epithelial Ovarian Cancer

Objective: Nude mice with orthotopic transplantation of human ovarian epithelial cancer were used toinvestigate screening criteria for paraneoplastic normal ovarian tissue and the security of the freezing andthawing for ovarian tissue transplantation.
Methods: Expression of CK-7, CA125, P53, survivin, MMP-2/TIMP-2 in paraneoplastic normal ovarian tissues were detected by RT-PCR as well as immunohistochemistry. Thetissues of the groups with all negative indicators of RT-PCR, all negative indicators of immunohistochemistry,negative expression of CK-7, CA125 and survivin, positive expression of CK-7, CA125 and survivin, cancertissues and normal ovarian tissues of nude mice were used for freezing and thawing transplantation, to analyzeovert and occult carcinogenesis rates after transplantation.
Results: When all indicators or the main indicators,CK-7, CA125 and survivin, were negative, tumorigenesis did not occur after transplantation. In addition theoccult carcinogenesis rate was lower than in the group with positive expression of CK-7, CA125 and survivin(P<0.01). After subcutaneous and orthotopic transplantation of ovarian tissues, rates did not change (P>0.05).There was no statistical significance among rates after transplantation of ovarian tissues which were obtainedunder different severity conditions (P>0.05).
Conclusion: Negative expression of CK-7, CA125 and survivin canbe treated as screening criteria for security of ovarian tissues for transplantation. Immunohistochemical methodscan be used as the primary detection approach. Both subcutaneous and orthotopic transplantation are safe. Theinitial severity does not affect the carcinogenesis rate after tissue transplantation. Freezing and thawing ovariantissue transplantation in nude mice with human epithelial ovarian carcinoma is feasible and safe.