Role of Integrin-Linked Kinase in Multi-drug Resistance of Human Gastric Carcinoma SGC7901/DDP Cells

Gastric carcinoma is a leading cause of cancer death in the world and multi-drug resistance (MDR) is anessential aspect of gastric carcinoma chemotherapy failure. Recent studies have shown that integrin-linked kinase(ILK) is involved in metastasis of human tumors, expression silencing of ILK inhibiting the metastasis of severaltypes of cultured human cancer cells. However, the role and potential mechanism of ILK to reverse the multidrugresistance in human gastric carcinoma is not fully clear. In this report, we focused on roles of expressionsilencing of ILK in multi-drug resistance reversal of human gastric carcinoma SGC7901/DDP cells, includingincreased drug sensitivity to cisplatin, cell apoptosis rates, and intracellular accumulation of Rhodamine-123,and decreased mRNA and protein expression of multi-drug resistance gene (MDR1), multi-drug resistanceassociatedprotein (MRP1), excision repair cross-complementing gene 1 (ERCC1), glutathione S-transferase -π(GST-π) and RhoE, and transcriptional activation of AP-1 and NF-κB in ILK silenced SGC7901/DDP cells. Wealso found that there was a decreased level of p-Akt and p-ERK. The results indicated that ILK might be used asa potential therapeutic strategy to combat multi-drug resistance through blocking PI3K-Akt and MAPK-ERKpathways in human gastric carcinoma.