Expression of hPOT1 in HeLa Cells and the Probability of Gene Variation of hpot1 Exon14 in Endometrial Cancer are Much Higher than in Other Cancers

To investigate the expression of hPOT1 in the HeLa cell line and screen point mutations of hpot1 in differenttumor tissues a two step osmotic method was used to extract nuclear proteins. EMSA was performed to determinethe expression of hPOT1 in the HeLa cell line. PCR was also employed to amplify the exon14 sequence of the hpot1gene in various of cancer tissues. A SV gel and PCR clean-up system was performed to enrich PCR products.DNAStar was used to analyse the exon14 sequence of the hpot1 gene. hPOT1 was expressed in the HeLa cell lineand the signal was gradually enhanced as the amount of extracted nuclear proteins increased. The DNA fragmentof exon14 of hpot1 was successfully amplified in the HeLa cell line and all cancer tissues, point mutations beingobserved in 2 out of 3 cases of endometrial cancer (66.7%) despite the hpot1 sequence being highly conserved.However, the sequence of hpot1 exon14 do not demonstrate point mutations in most cancer tissues. Since hPOT1was expressed in HeLa cell and the probability of gene point variants was obviously higher in endometrial cancerthan other cancers, it may be involved in the pathogenesis of gynecological cancers, especially in cervix andendometrium.