Ellagic Acid Inhibits Migration and Invasion by Prostate Cancer Cell Lines

Polyphenolic compounds from pomegranate fruit extracts (PFEs) have been reported to possessantiproliferative, pro-apoptotic, anti-inflammatory and anti-invasion effects in prostate and other cancers.However, the mechanisms responsible for the inhibition of cancer invasion remain to be clarified. In thepresent study, we investigated anti-invasive effects of ellagic acid (EA) in androgen-independent human(PC-3) and rat (PLS10) prostate cancer cell lines in vitro. The results indicated that non-toxic concentrationsof EA significantly inhibited the motility and invasion of cells examined in migration and invasion assays.The EA treatment slightly decreased secretion of matrix metalloproteinase (MMP)-2 but not MMP-9 fromboth cell lines. We further found that EA significantly reduced proteolytic activity of collagenase/gelatinasesecreted from the PLS-10 cell line. Collagenase IV activity was also concentration-dependently inhibited byEA. These results demonstrated that EA has an ability to inhibit invasive potential of prostate cancer cellsthrough action on protease activity.