Lentivirus Mediated GOLPH3 shRNA Inhibits Growth and Metastasis of Esophageal Squamous Cancer

Aim: To investigate the role of Golgi phosphoprotein 3 (GOLPH3) in tumour growth and metastasis ofesophageal squamous cancer.
Methods: A lentiviral shRNA-vector was utilized to stably knockdown GOLPH3in Eca-109 esophageal squamous cancer cells. mRNA transcription and protein expression of GOLPH3 wereexamined by real-time quantitative PCR and Western blotting, respectively. Cell proliferation activity was assessedby MTT assay and invasion and migration potentials by matrigel invasion and transwell motility assays.
Results:Stable knockdown in the GOLPH3 cell line was established. PD-A gene expression was significantly suppressedby lentivirus-mediated RNAi, which resulted in reducing the capacity for cell proliferation, migration, invasionand adhesion in vitro. In vivo, GOLPH3 depletion resulted in inhibition of tumour growth, with stable decreasein the expression of GOLPH3 in tumor xenografts.
Conclusions: Our findings suggest that lentivirus mediatedsilencing of the GOLPH3 gene has a significant anti-tumour effect on esophageal squamous cancer in vitro andin vivo. In addition, the results indicate that GOLPH3 might be an effective molecular target for gene therapyin esophageal squamous cancer.