Background: The two basic methods that are currently accepted to identify the HER2 status areimmunohistochemistry and flyorescence in situ hybridization (FISH) . The aim of this study was to perform thedual-color silver in situ hybridization (dc-SISH) technique as an alternative to FISH. Materials and Methods:A total of 40 invasive breast carcinoma cases were assessed for HER2 gene amplification by FISH and dualcolorSISH. Results: Significant correlation was found in the HER2 expression results obtained with the twoapproaches (p=0.001, p<0.05). The concordance rate was 92.3%. Conclusions: Foutine practical use of thedc-SISH method, which is much easier to apply, score, and evaluate, has many advantages. HER2 and CEN17status can be evaluated simultaneously with the newly developed “Dual-Color Probe”. All these specificationsand the reliable results obtained support the widespread use of SISH technique in clinical practice.
(2013). Determination of HER2 Gene Amplification in Breast Cancer using Dual-color Silver Enhanced in situ Hybridization (dc- SISH) and Comparison with Fluorescence ISH (FISH). Asian Pacific Journal of Cancer Prevention, 14(10), 6131-6134.
MLA
. "Determination of HER2 Gene Amplification in Breast Cancer using Dual-color Silver Enhanced in situ Hybridization (dc- SISH) and Comparison with Fluorescence ISH (FISH)". Asian Pacific Journal of Cancer Prevention, 14, 10, 2013, 6131-6134.
HARVARD
(2013). 'Determination of HER2 Gene Amplification in Breast Cancer using Dual-color Silver Enhanced in situ Hybridization (dc- SISH) and Comparison with Fluorescence ISH (FISH)', Asian Pacific Journal of Cancer Prevention, 14(10), pp. 6131-6134.
VANCOUVER
Determination of HER2 Gene Amplification in Breast Cancer using Dual-color Silver Enhanced in situ Hybridization (dc- SISH) and Comparison with Fluorescence ISH (FISH). Asian Pacific Journal of Cancer Prevention, 2013; 14(10): 6131-6134.
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