Introduction: Lung cancer is extremely harmful to human health and has one of the highest worldwideincidences of all malignant tumors. Approximately 80% of lung cancers are classified as non-small cell lungcancers (NSCLCs). Cisplatin-based multidrug chemotherapy regimen is standard for such lesions, but drugresistance is an increasing problem. F-box/WD repeat-containing protein 7 (FBW7) is a member of the F-boxprotein family that regulates cell cycle progression, and cell growth and differentiation. FBW7 also functions as atumor suppressor. Methods: We used cell viability assays, Western blotting, and immunofluorescence combinedwith siRNA interference or plasmid transfection to investigate the underlying mechanism of cisplatin resistancein NSCLC cells. Results: We found that FBW7 upregulation significantly increased cisplatin chemosensitivity andthat cells expressing low levels of FBW7, such as NCI-H1299 cells, have a mesenchymal phenotype. Furthermore,siRNA-mediated silencing or plasmid-mediated upregulation of FBW7 resulted in altered epithelial-mesenchymaltransition (EMT) patterns in NSCLC cells. These data support a role for FBW7 in regulating the EMT in NSCLCcells. Conclusion: FBW7 is a potential drug target for combating drug resistance and regulating the EMT inNSCLC cells.
(2013). FBW7 Upregulation Enhances Cisplatin Cytotoxicity in Nonsmall Cell Lung Cancer Cells. Asian Pacific Journal of Cancer Prevention, 14(11), 6321-6326.
MLA
. "FBW7 Upregulation Enhances Cisplatin Cytotoxicity in Nonsmall Cell Lung Cancer Cells". Asian Pacific Journal of Cancer Prevention, 14, 11, 2013, 6321-6326.
HARVARD
(2013). 'FBW7 Upregulation Enhances Cisplatin Cytotoxicity in Nonsmall Cell Lung Cancer Cells', Asian Pacific Journal of Cancer Prevention, 14(11), pp. 6321-6326.
VANCOUVER
FBW7 Upregulation Enhances Cisplatin Cytotoxicity in Nonsmall Cell Lung Cancer Cells. Asian Pacific Journal of Cancer Prevention, 2013; 14(11): 6321-6326.