Background: Nowadays, the encapsulation of cytotoxic chemotherapeutic agents is attracting interest as amethod for drug delivery. We hypothesized that the efficiency of helenalin might be maximized by encapsulationin β-cyclodextrin nanoparticles. Helenalin, with a hydrophobic structure obtained from flowers of Arnicachamissonis and Arnica Montana, has anti-cancer and anti-inflammatory activity but low water solubility andbioavailability. β-Cyclodextrin (β-CD) is a cyclic oligosaccharide comprising seven D-glucopyranoside units,linked through 1,4-glycosidic bonds. Materials and Methods: To test our hypothesis, we prepared β-cyclodextrinhelenalincomplexes to determine their inhibitory effects on telomerase gene expression by real-time polymerasechain reaction (q-PCR) and cytotoxic effects by colorimetric cell viability (MTT) assay. Results: MTT assayshowed that not only β-cyclodextrin has no cytotoxic effect on its own but also it demonstrated that β-cyclodextrinhelenalincomplexes inhibited the growth of the T47D breast cancer cell line in a time and dose-dependent manner.Our q-PCR results showed that the expression of telomerase gene was effectively reduced as the concentrationof β-cyclodextrin-helenalin complexes increased. Conclusions: β-Cyclodextrin-helenalin complexes exertedcytotoxic effects on T47D cells through down-regulation of telomerase expression and by enhancing Helenalinuptake by cells. Therefore, β-cyclodextrin could be superior carrier for this kind of hydrophobic agent.
(2013). Inhibitory Effects of β-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR. Asian Pacific Journal of Cancer Prevention, 14(11), 6949-6953.
MLA
. "Inhibitory Effects of β-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR". Asian Pacific Journal of Cancer Prevention, 14, 11, 2013, 6949-6953.
HARVARD
(2013). 'Inhibitory Effects of β-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR', Asian Pacific Journal of Cancer Prevention, 14(11), pp. 6949-6953.
VANCOUVER
Inhibitory Effects of β-Cyclodextrin-Helenalin Complexes on H-TERT Gene Expression in the T47D Breast Cancer Cell Line - Results of Real Time Quantitative PCR. Asian Pacific Journal of Cancer Prevention, 2013; 14(11): 6949-6953.