Experimental Study on Sustained-release 5-Fluorouracil Implantation in Canine Peritoneum and Para-aortic Abdominalis

Objective: To observe local and systemic toxicity after sustained-release 5-fluorouracil (5-Fu) implantationin canine peritoneum and para-aortic abdominalis and the changes of drug concentration in the local implantedtissue with time.
Methods: 300 mg sustained-release 5-Fu was implanted into canine peritoneum and para-aortaabdominalis. Samples were taken 3, 5, 7 and 10 days after implantation for assessment of changes and systemicreactions. High performance liquid chromatography was applied to detect the drug concentrations of peritonealtissue at different distances from the implanted site, lymphatic tissue of para-aortic abdominalis, peripheralblood and portal venous blood.
Results: 10 days after implantation, the drug concentrations in the peritoneum,lymphatic tissue and portal vein remained relatively high within 5 cm of the implanted site. There appearedinflammatory reaction in the local implanted tissue, but no visible pathological changes such as cell degenerationand necrosis, and systemic reaction like anorexia, nausea, vomiting and fever.
Conclusions: Sustained-release5-Fu implantation in canine peritoneum and para-aortic abdominalis can maintain a relatively high tumourinhibitingconcentration for a longer time in the local implanted area and portal vein, and has mild local andsystemic reactions. Besides, it is safe and effective to prevent or treat recurrence of gastrointestinal tumours andliver metastasis.