Sulforaphane Inhibits the Proliferation of the BIU87 Bladder Cancer Cell Line via IGFBP-3 Elevation

Aim: To investigate effects of sulforaphane on the BIU87 cell line and underlying mechanisms involvingIGFBP-3.
Methods: Both BIU87 and IGFBP-3-silenced BIU87 cells were treated with sulforaphane. Cellproliferation was detected by MTT assay. Cell cycle and apoptosis were determined via flow cytometry.Quantitative polymerase chain reaction and Western blotting were applied to analyze the expression of IGFBP-3and NF-κB at both mRNA and protein levels.
Results: Sulforaphane (80 μM) treatment could inhibit cellproliferation, inducing apoptosis and cell cycle arrest at G2/M phase. All these effects could be antagonized byIGFBP-3 silencing. Furthermore, sulforaphane (80 μM) could down-regulate NF-κB expression while elevatingthat of IGFBP-3.
Conclusions: Sulforaphane could suppress the proliferation of BIU87 cells via enhancingIGFBP-3 expression, which negatively regulating the NF-κB signaling pathway.