Asparagus Racemosus Leaf Extract Inhibits Growth of UOK 146 Renal Cell Carcinoma Cell Line: Simultaneous Oncogenic PRCCTFE3 Fusion Transcript Inhibition and Apoptosis Independent Cell Death

Abstract

Aims: To evaluate anti-cancer activity of Asparagus racemosus (AR) leaf extract on UOK146, a renal cellcarcinoma cell line, and explore its mechanism of action. Materials and
Methods: Dried AR leaves were extractedwith chloroform and dissolved in DMSO. This extract was applied to UOK146 and cell death was estimated byMTT assay. In addition PRCC-TFE3 fusion transcripts were detected by real time PCR.
Results: Extract wasfound to be cytotoxic with an IC50 of 0.9 mg/ml as estimated by dose response curve. Antitumor activity of thepermissible doses of the extract was assessed by the down regulation of PRCC-TFE3 fusion transcript (38%)responsible for oncogenicity of the UOK146 cell line. No increment in the BAX, a proapoptotic marker levelwas observed.
Conclusions: Evidence of antiproliferative effect, PRCC-TFE3 fusion transcript inhibition andstatic BAX level clearly indicate that AR extract provides or elicits an apoptosis independent anticancer effecton RCC cells by some specific mechanism of regulation.

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