Background: This study investigated the influence of curcumin on HOX transcript antisense RNA (HOTAIR)-mediated migration of cultured renal cell carcinoma (RCC) cells. Materials and Methods: Five RCC cell lines(769-P, 769-P-vector, 769-P-HOTAIR, 786-0, and Kert-3 ) were maintained in vitro. The expression of HOTAIRmRNA was determined by quantitative real-time PCR and cell migration was measured by transwell migrationassay. The effects of different concentrations of curcumin (0 to 80 μmol/L) on cell proliferation was determinedby the CCK-8 assay and influence of non-toxic levels (0 to 10 μM) on the migration of RCC cells was alsodetermined. Results: Comparison of the 5 cell lines indicated a correlation between HOTAIR mRNA expressionand cell migration. In particular, the migration of 769-P-HOTAIR cells was significantly higher than that of769-P-vector cells. Curcumin at 2.5-10 μM had no evident toxicity against RCC cells, but inhibited cell migrationin a concentration-dependent manner. Conclusions: HOTAIR expression is correlated with the migration ofRCC cells, and HOTAIR may be involved in the curcumin-induced inhibition of RCC metastasis.