Association of Cytokine Gene Polymorphisms with Gastritis in a Kazakh Population


Background: Gastritis and gastric cancer are the most common diseases in the Kazakh population.Polymorphisms in genes coding of cytokines have been played important role with gastric disease risk. Therisk alleles of cytokines in patients with gastritis can predict the risk of developing gastric cancer. The aimof this study was to investigate cytokine gene polymorphisms as risk factors for the development of gastritisin a case-control study with gastritis patients and healthy individuals from the Kazakh ethnic group, livingin North Kazakhstan. Materials and
Methods: The polymerase chain reaction followed by direct sequencingwere used for detection of two functional polymorphisms in the IL1 gene family, and TaqMan SNP GenotypingAssay Sets were applied for three potentially functional polymorphisms in the IL10 gene, and one in the TNFApromoter.
Results: Association analysis of studied allelic variants and the development of gastritis in H. pyloripositivepatients showed that IL1В -31С/С, IL1В -511T/T and IL1RN -2/2 allelic variants were associated withdevelopment of gastritis (OR=1.8 (1.07-3.16), p=0.025; OR=1.7 (1.04-2.99), p=0.035, and OR=4.92 (2.45-9.85),p<0.001) respectively. Haplotype С-Т that combines both homozygous allelic variants of IL1B gene also had astatistically significant association with slightly higher OR (OR: 1.43, 95% CI: 1.08-1.88).
Conclusions: The datafrom the current study showed that the genotype IL-1В -511Т/-31С-IL1-RN-2 and H. pylori infection increaserisk of gastritis in the Kazakh population. That genotype combination might be a factor increasing the risk ofdeveloping gastric cancer.