CYP2E1 Genetic Polymorphism with Dietary, Tobacco, Alcohol Habits, H. pylori Infection Status and Susceptibility to Stomach Cancer in Mizoram, India

Background: The incidence of stomach cancer in India is highest in the state of Mizoram. In this populationbased matched case-control study, we evaluated the relationship between CYP450 2E1 RsaI polymorphism andrisk of stomach cancer taking into considering various important dietary habits along with tobacco, alcoholconsumption and H. pylori infection status. Materials and
Methods: A total of 105 histologically confirmedstomach cancer cases and 210 matched healthy population controls were recruited. CYP2E1 RsaI genotypes weredetermined by PCR-RFLP and H. pylori infection status by ELISA. Information on various dietary, tobacco andalcohol habits was recorded in a standard questionnaire.
Results: Our study revealed no significant associationbetween the CYP2E1 RsaI polymorphism and overall risk of stomach cancer in Mizoram. However, we observeda non-significant protective effect of the variant allele (A) of CYP2E1 against stomach cancer. Tobacco smokerscarrying C/C genotype have three times more risk of stomach cancer, as compared to non-smokers carrying C/Cgenotype. Both Meiziol and cigarette current and past smokers who smoked for more than 10 times per day andcarrying the (C/C) genotype are more prone to develop stomach cancer. Smoke dried fish and preserved meat(smoked/sun dried) consumers carrying C/C genotype possesses higher risk of stomach cancer. No significantassociation between H. pylori infection and CYP2E1 RsaI polymorphism in terms of stomach cancer was observed.
Conclusions: Although no direct association between the CYP2E1 RsaI polymorphism and stomach cancer wasobserved, relations with different tobacco and dietary risk habits in terms of developing stomach cancer existin this high risk population of north-eastern part of India. Further in-depth study recruiting larger populationis required to shed more light on this important problem.