Expression of TS, RRM1, ERCC1, TUBB3 and STMN1 Genes in Tissues of Non-small Cell Lung Cancer and its Significance in Guiding Postoperative Adjuvant Chemotherapy


Background: To explore the expression of TS, RRM, ERCC1, TUBB3 and STMN1 genes in the tissues ofpatients with non-small cell lung cancer (NSCLC) and its significance in guiding the postoperative adjuvantchemotherapy. Materials and
Methods: Real time polymerase chain reaction (PCR) was applied to detect theexpression of TS, RRM, ERCC1, TUBB3 and STMN1 genes in the tissues of NSCLC patients so as to analyze therelationship between the expression of each gene and the clinical characteristics and to guide the postoperativeindividualized chemotherapy according to the detection results of NSCLC patients.
Results: Expression of TSgene was evidently higher in patients with adenocarcinoma than those with non-adenocarcinoma (P=0.013) andso was the expression of ERCC1 (P=0.003). The expression of TUBB3 gene was obviously higher in NSCLCpatients in phases Ⅰ/Ⅱ and Ⅳ than those in phase Ⅲ (P1=0.021; P2=0.004), and it was also markedly higher inpatients without lymph node metastasis than those with (P=0.008). The expression of STMN1 gene was apparentlyhigher in patients in phase Ⅰ/Ⅱ than those in phase Ⅳ (P=0.002). There was no significant difference betweenthe rest gene expression and the clinical characteristics of NSCLC patients (P>0.05). Additionally, the diseasefreesurvival (DFS) was significantly longer in patients receiving gene detections than those without (P=0.021).
Conclusions: The selection of chemotherapeutic protocols based singly on patients’ clinical characteristics hascertain blindness. However, the detection of tumor-susceptible genes can guide the postoperative adjuvantchemotherapy and prolong the DFS of NSCLC patients.