Chemopreventive Actions of Blond and Red-Fleshed Sweet Orange Juice on the Loucy Leukemia Cell Line


Background: Red-fleshed sweet orange juice (ROJ) comes from a new variety of citrus cultivated in Brazilthat contains high levels of β-carotene and lycopene, and similar amounts of hesperidin (HSP) and nutrients,equivalently to blond orange juice (BOJ). Such bioactive compounds are associated with chemopreventive actionsin several cancer cell lines. The purpose of this study was to examine the cytotoxicity, cell cycle, apoptosis, andcytokine secretion after BOJ, ROJ, and HSP treatment of a novel T acute lymphoblastic leukemia cell line,Loucy. Materials and
Methods: Loucy cells were incubated for 24-h with BOJ, ROJ, and HSP, and the viabilitywas measured using trypan blue. Cell cycling and apoptosis were assessed by propidium iodide (PI) and annexinV-FITC/PI flow cytometry, respectively. Secretion of cytokines IL-1α, IL1-β, IL-2, IL-4, IL-6, IL-10, IL-17A,IFNγ, TNFα, TGFβ, MIPα, and MIPβ was determined by ELISA array.
Results: BOJ and ROJ treatmentspromoted Loucy cell cytotoxicity. Additionally, BOJ induced cell cycle arrest in the G0/G1 phase, and decreasedthe cell accumulation in the G2/M. ROJ decreased only the G0/G1 fraction, while HSP did not change the cellcycle. BOJ led to apoptosis in a different fashion of ROJ, while the first treatment induced apoptosis by increaseof late apoptosis and primary necrotic fractions, the second increased early and late apoptosis, and primarynecrotic fraction compared to positive controls. HSP had no effect on apoptosis. IL-6 and IL-10 were abrogatedby all treatments.
Conclusions: Taking together, these results suggest potential chemopreventive effects of BOJand ROJ on Loucy cells.