Department of Hepatobiliary Surgery, the Affiliated Hospital of Guilin Medical College, Guilin, China
Abstract
Background: MicroRNAs (miRNAs) have fundamental roles in tumorigenesis. MiR-675 is upregulated in hepatocellular carcinoma(HCC) cells. However, the roles of miR-675 in hepatocellular carcinogenesis are still not fully elucidated. In this study, we focus on investigating the effect and mechanism of miR-675 in proliferation of HCC cells. Materials and Methods: The cell proliferation was measured by MTT assays after transfection with miR-675 inhibitor and miR-675 mimics in HCC cells. The expression level of miR-675 was detected by real-time quantitative reverse transcription polymerase chain reaction. Protein expression of Cdc25A was measured by western blotting analysis. Results: In MTT assays, overexpression of miR-675 promoted the proliferation of HCC cells(PPPPp<0.05) after treatment with miR-675 mimics. The expression level of Cdc25A was significantly decreased (p<0.05) after treatment with miR-675 inhibitor. Conclusions: Our results indicate that miR-675 promotes the proliferation in human hepatocellular carcinoma cells by associating with Cdc25A signaling pathway.
Yu, Y., Weng, J., Li, S., Li, B., & Lv, J. (2016). MiR-675 Promotes the Growth of Hepatocellular Carcinoma Cells Through Cdc25A Pathway. Asian Pacific Journal of Cancer Prevention, 17(8), 3881-3885.
MLA
Ya-Qun Yu; Jun Weng; Shu-Qun Li; Bo Li; Jun Lv. "MiR-675 Promotes the Growth of Hepatocellular Carcinoma Cells Through Cdc25A Pathway". Asian Pacific Journal of Cancer Prevention, 17, 8, 2016, 3881-3885.
HARVARD
Yu, Y., Weng, J., Li, S., Li, B., Lv, J. (2016). 'MiR-675 Promotes the Growth of Hepatocellular Carcinoma Cells Through Cdc25A Pathway', Asian Pacific Journal of Cancer Prevention, 17(8), pp. 3881-3885.
VANCOUVER
Yu, Y., Weng, J., Li, S., Li, B., Lv, J. MiR-675 Promotes the Growth of Hepatocellular Carcinoma Cells Through Cdc25A Pathway. Asian Pacific Journal of Cancer Prevention, 2016; 17(8): 3881-3885.
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