Antioxidant and Antiproliferative Activities of an Ethylacetate Fraction of Picria Fel-Terrae Lour. Herbs

Document Type : Research Articles


1 Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Sumatera Utara

2 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Sumatera Utara

3 Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara

4 Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara


Background: Excessive production of oxygen free radicals and imbalance in the mechanisms responsible for antioxidant protection may result in the onset of many diseases including breast cancer. Objective: To evaluate antiproliferative and antioxidant activity of an ethylacetate fraction (EAF) of Picria fel-terrae Lour. Herbs in the T47D cell line. Methods: Phenolic and total flavonoid contents in EAF were determined. EAF was tested for cytotoxicity and effects on the cell cycle and apoptosis, as well as antioxidant activity. Results: EAF was found to contain high levels of phenolic agents (92.88 ± 0.50 mg GAE/g), total flavonoid (84.39 ± 0.07 mg QE/g). The EAF of Picria fel-terrae Lour. herbs was found to have an IC50 of 62.98μg/mL, caused accumulation in G0-G1 and S phase and increased early and late apoptosis. Antioxidant activity in DPPH assays gave an IC50 of 166.90 ± 0.10 μg/mL. Conclusions: The results reveal that EAF of Picria fel-terrae Lour. herbs has antiproliferative activity and strong antioxidant potential. Further studies are now needed to isolate the responsible antiproliferative and antioxidant components.


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