Effects of Tobacco Habits on the Polymorphism of NFKB1 and NFKB1A Gene of Head and Neck Squamous Cell Carcinoma in Indian Population

Gupta, Abhishek; Agnihotri, Vertica; Kumar, Rahul; Upadhyay, Ashish; Bhaskar, Suman; Dwivedi, Sadanand; Dey, Sharmistha

doi:10.22034/APJCP.2017.18.7.1855

Effects of Tobacco Habits on the Polymorphism of NFKB1 and NFKB1A Gene of Head and Neck Squamous Cell Carcinoma in Indian Population

Document Type : Research Articles

Authors

¹ Department of Biophysics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.

² Department of Biostatistics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.

³ Department of Radiation Oncology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.

10.22034/APJCP.2017.18.7.1855

Abstract

Background: Polymorphism of NFKB1 and NFKB1A are highly associated with cancer. We have assessed polymorphism in the promoter region of NFKB1 -94 del/ins ATTG (rs28362491) and NFKB1A -826 C/T (rs2233406) with the risk of HNSCC in Indian population. Methods: Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method was used for the genotyping NFKB1 -94 del/ins ATTG and NFKB1A -826 C/T. Sequencing was done to validate the results of PCR-RFLP. Statistical analysis of data was done by Stata/SE-14.0 software. Results: ins/ins genotype was observed to be a risk factor of HNSCC as compared del/del genotype of NFKB1 -94 ATTG. Interactive effects of smoking and chewing on ins/ins genotype showed 13.96 and 10.92 fold increased risk of HNSCC. NFKB1A -826 C/T polymorphism, TT genotype showed no association with the risk of HNSCC as compared to wild type CC genotype. Conclusion: Our results showed NFKB1 -94 del/ins ATTG with smoking and tobacco chewing may increase the risk of HNSCC while NFKB1A -826 C/T plays a protective role in Indian population.

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