Antimigration Activity of an Ethylacetate Fraction of Zanthoxylum acanthopodium DC. Fruits in 4T1 Breast Cancer Cells

Document Type : Research Articles


1 Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Indonesia.

2 Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Sumatera Utara, Indonesia.

3 Department of Anatomy, Faculty of Medicine, Gadjah Mada University, Indonesia.


Objective: This study was carried out to investigate the antimigration activity of Zanthoxylum acanthopodium DC.
in the 4T1 breast cancer cell line. Methods: Zanthoxylum acanthopodium DC. fruit powder was extracted by maceration
method with n-hexane and ethylacetate solvents. Cytotoxicity and proliferation were assessed using the MTT method
and the cell cycle by flow cytometry. In addition, wound healing assays were conducted by a microscopic method,
and expression of COX-2 and VEGFR-2 were determined using qRT-PCR. Results: The IC50 of the ethylacetate
fraction (EAF) was 48.1 ± 1.06 μg/mL. The EAE at a concentration 10 μg/mL with viable cells was 62.3 ± 0.28%
after 72 h incubation, with accumulation in the G2-M phase, inhibition of cell migration in the wound healing assay,
and decrease in expression of COX-2 (0.62 ± 0.01) and VEGFR-2 (0.39 ± 0.003). Conclusion: The results reveal that
an ethylacetate fraction of Zanthoxylum acanthopodium DC. fruits provides effective antimigration effects. Further
studies are now planned to assess the potential of the ethylacetate fraction to inhibit angiogenesis in breast cancer and
determine underlying mechanisms.


Main Subjects