Adverse Effects of Vincristine Chemotherapy on Cell Changes in Seminiferous Tubules and Cetrorelix GnRH Antagonist Inhibitory Effects in Mice

Document Type : Research Articles


1 Department of Histological Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.

2 Department of Anatomical Science, Tabriz University of Medical Sciences, Tabriz, Iran.

3 Department of Medical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.


The present study was designed to determine changes in spermatogenesis in adult mice after an intraperitoneal injection
of vincristine. The effect of a GnRH antagonist synchronous to chemotherapy, which might protect spermatogenesis
by halting cell division in spermatogenic cells, was also investigated. Method and Materials: A total of 30 adult male
mice were studied in three equal groups of ten. In the V group, a single dose of the chemotherapy drug vincristine was
injected intraperitonally at 1.5 mg/kg. In the V+C, group, the injection of Cetrorelix was started, and one week before
to one week after vincristine injection continued ( for 3 weeks). Controls received no treatment. Samples were taken
from the testicles, and fixed in Boueins fixative for light microscopy. Results: Comparing the mean number of Sertoli
and spermatogony cells and the rate of spermatogenesis index (SI) in the V group with controls showed significant
differences,which were not evident in the V+C group. Conclusion: According to the results, the cetrorelix antagonist
(GnRH) could largely prevent side effects of vincristine administration regarding seminiferous tubules.


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