Levels of Pro and Anti-inflammatory Citokynes and C-Reactive Protein in Patients with Chronic Periodontitis Submitted to Nonsurgical Periodontal Treatment

Document Type : Research Articles


1 University of Uberaba, Uberaba, MG, Brazil.

2 Cefores, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.

3 Centro Universitário do Triângulo, Uberlândia, MG, Brazil.

4 São Leopoldo Mandic Institute and Research Center, Campinas, SP, Brazil.


Aim: to compare the levels of IFN-γ, TGF-β and C-reactive protein (CRP) in healthy patients (HP) and chronic
periodontitis patients (CP) before and seven days after the last session of Non-Surgical Periodontal Treatment (NSPT).
Materials and Methods: 40 subjects were divided into two groups: healthy (n= 20), and with chronic periodontitis
(n = 20). Serum and gingival crevicular fluid (GCF) were collected from each patient and quantified for IFN-γ,
TGF-β and CRP using the enzyme-linked immunosorbent assay (ELISA). Results: IFN-γ was found to be higher in
the GCF of the CP group before NSPT in relation to the HP group (p<0.05), and it had significant higher levels after
seven days of NSPT (p<0.05). The levels of TGF-β in the GCF of CP patients before NSPT were significantly higher
when compared to HP (p<0.05), but they decreased after seven days of NSPT (p>0.05). Serum CRP levels did not show
statistical difference between CP and HP before or after NSPT. Conclusion: Therefore, our results demonstrated for
the first time that NSPT causes early exacerbation of the immune response at the local level represented by increased
levels of IFN-γ and decreased levels of TGF-β in the gingival crevicular fluid after seven days of treatment.


Main Subjects