Analysis of Polymorphism and Expression Profile of ASIC1 and IL-6 Genes in Patients with Gastric Cancer

Document Type : Research Articles


1 Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran.

2 Department of Radiation Oncology, Isfahan University of Medical Science, Isfahan, Iran.


Abstract: Gastric cancer is one of the most common upper gastrointestinal malignancies. Some Iranian provinces,
such as in the northern and northwestern areas, are at a high risk, whereas the central and western provinces are at
a medium and the southern regions at low risk. This study was carried out to estimate the impact of the expression
patterns of ASIC1 and IL-6 genes and the IL-6rs-174 and ASIC1rs 75624685 polymorphisms in the pathogenesis of
gastric cancer. Materials and methods: Tetra-ARMS PCR was employed to analyze the polymorphism status of the
ASIC1 and IL-6 genes with 85 paraffin-embedded tissue blocks from cases and 117 normal blood samples as controls.
We also investigated mRNA expression levels of these genes in 12 cases and controls using real-time PCR. Results:
Our results showed a significant association between expression of ASIC1 and elevated risk of gastric cancer (p<0.001).


Main Subjects