Document Type : Research Articles
Authors
1
Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran.
2
Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
3
Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran.
4
Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran.
5
Department of Biology, Science and Research Branch, Islamic Azad University , Tehran, Iran.
6
Faculty of Health, Qom University of Medical Sciences, Qom, Iran.
7
Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran.
8
Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
Abstract
Background: Drug delivery systems have been designed to achieve targeted delivery and control the release rate
of the drugs. A serious challenge associated with drug delivery systems is the presence of the blood-brain barrier which
limits drugs penetration. In the current study, the effects of cisplatin nanoparticles on A172 brain cancer cell line were
investigated. Methods: Cisplatin nanoparticles were produced by miniemulsion polymerization technique and their
properties were evaluated. Drug release assay was performed to characterize the nanoparticles’ properties. Here, we
examined the effects of cisplatin nanoparticles and free form of cisplatin on A172 cancer cell line. MTT assay was
performed for different concentrations of the drug. To measure the apoptosis rate in A172 cell line in the presence
of cisplatin nanoparticles or its free from, Annexin V staining method was used. Results: Our results indicated that
loading type of cisplatin was physical loading and only 4.7% of cisplatin was released after 68 h. Furthermore, MTT
assay showed that cisplatin nanoparticles in all concentrations had more cytotoxic effects on the cells comparing with
the free form of cisplatin and control groups. We also showed that cisplatin nanoparticles could increase apoptosis
in cancer cells more than the drug in the free form by using flow cytometry technique. Conclusion: Overall, these
findings proved that cisplatin loaded on poly (Butylcyanoacrylate) nanoparticles, was more efficient than the free form
of cisplatin in treating A172 cancer cell line.
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