Plasma Peptidome Pattern of Breast Cancer Using Magnetic Beads-Based Plasma Fractionation and MALDI-TOF MS: A Case Control Study in Egypt

Document Type : Research Articles

Authors

1 Department of Chemical Pathology, Medical Research Institute, Alexandria University, Egypt.

2 Department of Clinical Pathology, Faculty of Medicine, Alexandria University, Egypt.

3 Department of Experimental and Clinical Surgery, Medical Research Institute, Alexandria University, Egypt.

Abstract

Objective: The present study aimed to determine peptidome patterns in breast cancer (BC). Methods: We analyzed
the plasma proteomic profiling of 80 BC patients and 50 healthy controls, using hydrophobic interaction chromatography
magnetic beads (MB-HIC8) separation followed by Matrix assisted laser desorption ionization/ time of flight mass
spectrometry (MALDI-TOF MS). Results: ClinProTools software identified 92 peaks that differed among the analyzed
groups, 33 peaks were significantly different (P < 0.05). Of those, 22 peaks were up-regulated while 11 peaks were
down-regulated in BC patients compared with the healthy controls. Three peptide ion signatures (m/z 1,570.31, 1,897.4
and 2,568.17) were provided by the Quick Classifier model to discriminate BC patients from healthy control subjects
with 96.4% accuracy. External validation was performed by an independent group and this achieved a sensitivity of
100% and a specificity of 76.9%. Conclusion: MALDI-TOF MS has good analytical performance in distinguishing
BC patients from healthy controls.

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Main Subjects

Asian Pacific Journal of Cancer Prevention
Volume 20, Issue 1
January 2019
Pages 175-184

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History

  • Receive Date: 15 July 2018
  • Revise Date: 06 November 2018
  • Accept Date: 30 December 2018

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