Regulatory Effect of Resveratrol and Prednisolone on MDR1 Protein Expression in Acute Lymphoblastic Leukemia Cell Line (CCRF-CEM)

Document Type : Research Articles


1 Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

2 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

3 Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.

4 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

5 Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

6 Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.

7 Medicinal Plants Research Center, Yasuj University of Medical sciences, Yasuj, Iran.


Objective: Chemotherapy is the most widely recognized technique to regard leukemia and also different sorts of
human tumors. In any case, tranquilize protection has stayed as the primary test against the adequacy of medications.
Besides, having different unfriendly impacts, chemotherapy drugs are getting to be traded by characteristic modalities for
growth treatment. In such manner, natural segments, for example, resveratrol and prednisolone have been recognized to
sharpen the leukemic cells to modified cell demise through an arrangement of complex procedures. In this investigation,
we have analyzed effect of 15, 50 and 100μM of resveratrol and 700μM of prednisolone on the human multidrug
protection quality 1 (MDR1) as a notable marker for cell sedate protection. We assessed the impact of resveratrol and
prednisolone on MDR1 protein expression in the CCRF-CEM cell line as an agent for intense lymphoblastic leukemia.
The investigation was planned to clear up whether. Materials and methods: CCRF-CEM cells linage get under
drug treatment with use of resveratrol and prednisolone. Western blot use at 24 and 48 hours with different doses of
resveratrol and prednisolone to analysis of MDR1 expression changes. Results: Effect of 15, 50, and 100 micro molar
of resveratrol and 700 micro molars of prednisolone on CCRF-CEM cells led to the MDR1 decrease. Western blot use
for evaluation of MDR1 protein expression changes. Conclusion: In the present study, we observed that resveratrol
and prednisolone, with a dose-dependent effect, can reduce the expression of the MDR1 protein. This reduction of
expression demonstrates that resveratrol and prednisolone can overcome to drug resistance created by MDR1.


Main Subjects

Volume 20, Issue 4
April 2019
Pages 1171-1176
  • Receive Date: 06 October 2018
  • Revise Date: 07 December 2018
  • Accept Date: 21 January 2019
  • First Publish Date: 01 April 2019