The New Compound of (2R, 4S)-N-(2, 5-difluorophenyl)-4- Hydroxy-1-(2, 2, 2-Trifluoroacetyl) Pyrrolidine-2-Carboxamide to Mediate the Expression of Some Apoptosis Genes by the HepG2 Cell Line

Document Type : Research Articles


1 Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

2 Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Department of Chemistry, Faculty of Science, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran.

4 Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

5 Department of Clinical Biochemistry, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.


Objectives: Hepatocellular carcinoma is one of the most frequent cancers worldwide, for the treatment of which
various therapy protocols and drugs have been introduced; however, none of them has suppressed cancer tissues
completely. New research programs have been developed on cancer and the accompanied effects of novel synthesized
compounds on cancer cell lines. Our latest reports on the molecular basis of cancer revealed a pattern of changes in
gene expression triggered in the cancer pathway. Methods: HepG2 cell lines were cultured under similar conditions
in both test and control groups. The IC50 concentration of the (2R, 4S)-N-(2, 5-difluorophenyl)-4-hydroxy-1-(2, 2,
2-trifluoroacetyl) pyrrolidine-2-carboxamide compound was used in the treatment group. After 48 hours from the
culture, the expressional profiles of apoptosis pathway genes (84 genes) were studied using the PCR array method.
Results: The findings demonstrated that the expression of some apoptosis-related genes pertaining to TNF, BCL2,
IAP, and caspase families was regulated by (2R, 4S)-N-(2, 5-difluorophenyl)-4-Hydroxy-1-(2, 2, 2-Trifluoroacetyl)
Pyrrolidine-2-Carboxamide. In the same vein, an alteration was observed in the expression of both pro-apoptotic and
anti-apoptotic genes associated with the extrinsic and intrinsic apoptosis signaling pathways. Conclusions: According
to the data obtained, the pyrrolidine-2-carboxamide compound was demonstrated to be able to regulate the apoptotic
activities of HepG2 cells by affecting both pro-apoptotic and anti-apoptotic relevant genes.


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