Effects of Mangaba (Hancornia speciosa) Fruit Extract Adsorbed onto PEG Microspheres in MCF-7 Breast Cancer Cells Co-Cultured with Blood Cells

De Araújo, Renata Lázara; Savazzi, Suiane; Fujimori, Mahmi; Deluque, Alessandra; Honório-França, Eduardo Luzia; Pertuzatti Konda, Paula Becker; Honório-França, Adenilda Cristina

doi:10.31557/APJCP.2019.20.7.1995

Effects of Mangaba (Hancornia speciosa) Fruit Extract Adsorbed onto PEG Microspheres in MCF-7 Breast Cancer Cells Co-Cultured with Blood Cells

Document Type : Research Articles

Authors

Renata Lázara de Araújo ¹
Suiane Savazzi ¹
Mahmi Fujimori ²
Alessandra Deluque ²
Eduardo Luzia Honório-França ¹^{, 2}
Paula Becker Pertuzatti Konda ¹
Adenilda Cristina Honório-França ¹^{, 2}

¹ Post Graduate Program in Material Science, Federal University of Mato Grosso, Barra do Garças, MT, Brazil.

² Institute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças, MT, Brazil.

10.31557/APJCP.2019.20.7.1995

Abstract

Objective: To evaluate the antitumor effects of polyethylene glycol (PEG) microspheres with adsorbed Hancornia
speciosa ethanolic extract (HSEE) on blood mononuclear (MN) cells co-cultured with MCF-7 breast cancer cells.
Methods: PEG microspheres were adsorbed with HSEE and examined by flow cytometry and fluorescence microscopy.
MCF-7 and MN cells obtained from volunteer donors were pre-incubated alone or co-cultured (MN and MCF-7
cells) for 24 h with or without HSEE, PEG microspheres or PEG adsorbed with HSEE (PEG-HSEE). Cell viability,
superoxide release and superoxide dismutase were determined. Results: Fluorescence microscopy showed that PEG
microspheres were able to absorb HSEE throughout their surface. Irrespective of the treatment, the viability index of
MN cells, MCF-7 and their co-culture was not affected. Superoxide release increased in co-cultured cells treated with
HSEE, adsorbed or not onto PEG microspheres. In co-cultured cells, SOD levels in culture supernatant increased in
the treatment with HSEE, adsorbed onto PEG microspheres or not. Conclusion: HSEE has direct effects on MN cells
co-cultured with MCF-7 cells. The results suggest the benefits of Hancornia speciosa fruit consumption by women at
risk of breast cancer. In addition, because PEG-HSEE maintained oxidative balance in co-cultured cells, it is a promising
alternative for the treatment of tumor cells.

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