Tissue P16 is Associated with Smoking Status among Indonesian Nasopharyngeal Carcinoma Subjects

Document Type : Research Articles


1 Department of Anatomical Pathology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada (FK-KMK UGM), Yogyakarta, Indonesia.

2 Faculty of Medicine, Universitas Muhammadiyah, Surakarta, Indonesia.

3 Department of Otorhinolaryngology Head and Neck Surgery, FK-KMK UGM, Yogyakarta, Indonesia.

4 Department of Histology and Cell Biology, FK-KMK UGM, Yogyakarta, Indonesia.


Background: Nasopharyngeal carcinoma (NPC) is a malignancy with high incidence in Southern China and
South-East Asia. NPC incidence among males in Indonesia is estimated around 8.3/100,000 populations. Tobacco
smoking is a common risk factor for cancer, including NPC. P16 is one of the key proteins related to the activation of
apoptotic pathways, that commonly change during carcinogenesis. Carcinogenesis is often related to environmental
exposure, including tobacco smoke. Objective: To analyze the association between P16 protein and smoking status
among NPC subjects in Indonesia. Methods: Forty formalin fixed-paraffin embedded NPC tissue samples of known
smoking status (20 smokers, 20 non-smokers) were collected from the Department of Anatomical Pathology, Dr. Sardjito
Hospital, Yogyakarta. P16 was detected by immunohistochemistry staining. German semi-quantitative scoring system
was applied to the P16 staining. Expression index with the score of 0 to 3 was classified as negative staining, meanwhile
4 to 12 was classified as positive staining. The association between P16 (score) and smoking status among NPC patients
was analyzed using Fischer exact test. One-sided p ≤ 0.05 was considered as statistically significant. Results: All
samples were Javanese males, with age range 25-76 years old. P16 positive staining among smokers was 5% (1/20),
while among non-smokers was 40% (8/20). P16 among smokers was significantly lower than non-smokers patients
(p=0.010). No difference was found between quantity of smoke and P16 score. Conclusion : A significant association
between P16 and smoking status in Indonesian NPC patients has been revealed. The result of this study may be used
to improve prevention and management of NPC cases related to smoking habit in Indonesia.


Main Subjects