Liver Pathology in Rats Treated with Newcastle Disease Virus Strains AF2240 and V4-UPM

Document Type : Research Articles


1 Department of Microbiology and Immunology, Faculty of Medicine and Health Sciences, Sana’a University, Sana'a, Yemen.

2 Faculty of dentistry, MAHSA University, Jenjarom, Malaysia.

3 Department of Pathology, Faculty of Medicine, Universiti Sultan Zainal Abidin, Kota Campus, Terengganu, Malaysia.

4 Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin,Kota Campus, Terengganu, Malaysia.


Background: Treatment of cancer with chemo-radiotherapy causes severe side effects due to cytotoxic effects
towards normal tissues which often results in morbidity. Therefore, developing anticancer agents which can selectively
target the cancer cells and cause less side effects are the main objectives of the new therapeutic strategies for treatment
advanced or metastatic cancers. Newcastle disease virus strains AF2240 and V4-UPM were shown to be cytolytic
against various cancer cells in-vitro and very effective as antileukemicagents. Methods: 45 rats at 6 weeks of age, were
randomly assigned to nine groups with 5 rats in each group, both azoxymethane (AOM) and 5-Fluorouracil (5-FU)
were given to rats according to the body weight. NDV virus strains (AF2240 and V4-UPM) doses were determined to
rats according to CD50 resulted from MTT assay. After 8 doses of NDV strians and 5-FU, tissue sections preparations
and histopathological study of rats’ organs were done. Results: In this article morphological changes of rats’ organs,
especially in livers, after treatment with a colon carcinogen (azoxymethane) and Newcastle disease virus strains
have been recorded. We observed liver damage caused by AOM evidenced by morphological changes and enzymatic
elevation were protected by the oncolytic viruses sections. Also we found that combination treatment NDV with 5-FU
had greater antitumor efficacy than treatment with NDV or 5-FU alone. Conclusion: We noted morphological changes
in liver and other rats’ organs due to a chemical carcinogen and their protection by NDV AF2240 and NDV V4-UPM
seems to be most protective.


Main Subjects