Background: Altered metabolism is one of the hallmarks of the cancer cells which reciprocally interrelate with epigenetic processes, such as post-translational histone modifications to maintain their desired gene expression profiles. The role of beta-hydroxybutyrate as a ketone body in cancer cell biology and histone modifications are reported. The present study aimed to evaluate the impacts of long-term metabolic reprogramming via glucose restriction and beta-hydroxybutyrate treatment on histone acetylation and butyrylation in MDA-MB231 cells as a model of triple negative stem-like breast cancer. Methods: For long-term treatment, cells were set up in three groups receiving DMEM with restricted glucose (250 mg/L), DMEM with restricted glucose but enriched with five millimolar beta-hydroxybutyrate and DMEM with standard glucose (1g\L) and investigated for a month. Histone modifications, including H3 acetylation and butyrylation, were investigated by immunoblotting after an acid extraction of the histone proteins. Results and Conclusion: Neither beta-hydroxybutyrate enrichment nor glucose restriction elicited a significant effect on the butyrylation or acetylation level of histone H3 upon a long-term treatment. Metabolic plasticity of cancer cells, mainly stem-like triple negative breast cancer cells alleviate or neutralize the impact of long-term metabolic reprogramming via restriction of glucose and histone modifications enrichment. These results shed new light upon the mechanism of controversial efficacy of ketogenic diets in clinical trials.
Mehdikhani, F., Ghahremani, H., Nabati, S., Tahmouri, H., Sirati-Sabet, M., & Salami, S. (2019). Histone Butyrylation/ Acetylation Remains Unchanged in Triple Negative Breast Cancer Cells after a Long Term Metabolic Reprogramming. Asian Pacific Journal of Cancer Prevention, 20(12), 3597-3601. doi: 10.31557/APJCP.2019.20.12.3597
MLA
Fatemeh Mehdikhani; Hossein Ghahremani; Saeedeh Nabati; Hanieh Tahmouri; Majid Sirati-Sabet; Siamak Salami. "Histone Butyrylation/ Acetylation Remains Unchanged in Triple Negative Breast Cancer Cells after a Long Term Metabolic Reprogramming". Asian Pacific Journal of Cancer Prevention, 20, 12, 2019, 3597-3601. doi: 10.31557/APJCP.2019.20.12.3597
HARVARD
Mehdikhani, F., Ghahremani, H., Nabati, S., Tahmouri, H., Sirati-Sabet, M., Salami, S. (2019). 'Histone Butyrylation/ Acetylation Remains Unchanged in Triple Negative Breast Cancer Cells after a Long Term Metabolic Reprogramming', Asian Pacific Journal of Cancer Prevention, 20(12), pp. 3597-3601. doi: 10.31557/APJCP.2019.20.12.3597
VANCOUVER
Mehdikhani, F., Ghahremani, H., Nabati, S., Tahmouri, H., Sirati-Sabet, M., Salami, S. Histone Butyrylation/ Acetylation Remains Unchanged in Triple Negative Breast Cancer Cells after a Long Term Metabolic Reprogramming. Asian Pacific Journal of Cancer Prevention, 2019; 20(12): 3597-3601. doi: 10.31557/APJCP.2019.20.12.3597
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