Background: Altered metabolism is one of the hallmarks of the cancer cells which reciprocally interrelate with epigenetic processes, such as post-translational histone modifications to maintain their desired gene expression profiles. The role of beta-hydroxybutyrate as a ketone body in cancer cell biology and histone modifications are reported. The present study aimed to evaluate the impacts of long-term metabolic reprogramming via glucose restriction and beta-hydroxybutyrate treatment on histone acetylation and butyrylation in MDA-MB231 cells as a model of triple negative stem-like breast cancer. Methods: For long-term treatment, cells were set up in three groups receiving DMEM with restricted glucose (250 mg/L), DMEM with restricted glucose but enriched with five millimolar beta-hydroxybutyrate and DMEM with standard glucose (1g\L) and investigated for a month. Histone modifications, including H3 acetylation and butyrylation, were investigated by immunoblotting after an acid extraction of the histone proteins. Results and Conclusion: Neither beta-hydroxybutyrate enrichment nor glucose restriction elicited a significant effect on the butyrylation or acetylation level of histone H3 upon a long-term treatment. Metabolic plasticity of cancer cells, mainly stem-like triple negative breast cancer cells alleviate or neutralize the impact of long-term metabolic reprogramming via restriction of glucose and histone modifications enrichment. These results shed new light upon the mechanism of controversial efficacy of ketogenic diets in clinical trials.
Mehdikhani, F. , Ghahremani, H. , Nabati, S. , Tahmouri, H. , Sirati-Sabet, M. and Salami, S. (2019). Histone Butyrylation/ Acetylation Remains Unchanged in Triple Negative Breast Cancer Cells after a Long Term Metabolic Reprogramming. Asian Pacific Journal of Cancer Prevention, 20(12), 3597-3601. doi: 10.31557/APJCP.2019.20.12.3597
MLA
Mehdikhani, F. , , Ghahremani, H. , , Nabati, S. , , Tahmouri, H. , , Sirati-Sabet, M. , and Salami, S. . "Histone Butyrylation/ Acetylation Remains Unchanged in Triple Negative Breast Cancer Cells after a Long Term Metabolic Reprogramming", Asian Pacific Journal of Cancer Prevention, 20, 12, 2019, 3597-3601. doi: 10.31557/APJCP.2019.20.12.3597
HARVARD
Mehdikhani, F., Ghahremani, H., Nabati, S., Tahmouri, H., Sirati-Sabet, M., Salami, S. (2019). 'Histone Butyrylation/ Acetylation Remains Unchanged in Triple Negative Breast Cancer Cells after a Long Term Metabolic Reprogramming', Asian Pacific Journal of Cancer Prevention, 20(12), pp. 3597-3601. doi: 10.31557/APJCP.2019.20.12.3597
CHICAGO
F. Mehdikhani , H. Ghahremani , S. Nabati , H. Tahmouri , M. Sirati-Sabet and S. Salami, "Histone Butyrylation/ Acetylation Remains Unchanged in Triple Negative Breast Cancer Cells after a Long Term Metabolic Reprogramming," Asian Pacific Journal of Cancer Prevention, 20 12 (2019): 3597-3601, doi: 10.31557/APJCP.2019.20.12.3597
VANCOUVER
Mehdikhani, F., Ghahremani, H., Nabati, S., Tahmouri, H., Sirati-Sabet, M., Salami, S. Histone Butyrylation/ Acetylation Remains Unchanged in Triple Negative Breast Cancer Cells after a Long Term Metabolic Reprogramming. Asian Pacific Journal of Cancer Prevention, 2019; 20(12): 3597-3601. doi: 10.31557/APJCP.2019.20.12.3597
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