Apoptosis Induced by Prednisolone Occurs without Altering the Promoter Methylation of BAX and BCL-2 Genes in Acute Lymphoblastic Leukemia Cells CCRF-CEM

Document Type : Research Articles


1 School of Medicine, Karazin Kharkiv National University, Kharkiv, Ukraine.

2 Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.

3 Medicinal Plants Research Center, Yasuj University of Medical sciences, Yasuj, Iran.

4 Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

5 Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.

6 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

7 Student Research Committee, Maragheh University of Medical Sciences, Maragheh, Iran.


Objective: one of the main mechanisms in which cancer cells are resistant to chemotherapy drugs and therapeutic strategies is resistance to apoptosis due to these anticancer factors. Regulating the expression of genes through epigenetics, especially regulation through methylation, is one of the key aspects of regulating gene expression and the function of genes, which is also regulated by the pathways regulating the pathway of apoptosis. The epigenetic regulatory phenomenon in cancer cells can undergo a change in regulation and induces resistance to apoptosis against chemotherapy and anticancer factors. The purpose of the present scrutiny was defined to probe the effect of subtoxic prednisolone dose on the level of promoter methylation and gene expression of BAX and BCL2 in the CCRF-CEM cells. Methods: The treated cells by prednisolone, cultured in RPMI 1640 medium in standard condition. Alteration in promoter DNA methylation was analyzed by use of methylation specific-PCR (MSP) technique after the defined intervened time of Prednisolone treatment with a subtoxic dose. Results: Prednisolone can induce apoptosis via alteration in BAX and BCL2 genes, based on our previous scrutiny. This essay shows no varies in the Pattern of DNA methylation of examined genes; however, prednisolone changes the expression of examined genes. Conclusion: Lack of alteration through prednisolone treatment in DNA methylation template of BAX and BCL2 genes make this possible that Prednisolone affects apoptotic gene expression via different pathways, which need more research to be done about it.


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