Genomic Instability in Peripheral Blood and Buccal Mucosal Cells of Marijuana Smokers: The Impact of Tobacco Smoke

Document Type : Research Articles


1 Department of Biosciences, Federal University of Sao Paulo, UNIFESP, Santos, SP, Brazil.

2 University São Judas Tadeu, Campus UNIMONTE, Santos, SP, Brazil.


Background: The aim of this study was to evaluate cytotoxic, mutagenic and genotoxic effects on buccal mucosa and peripheral blood cells from marijuana and tobacco smokers. Methods: For this purpose, a total of 45 volunteers were distributed into four groups: CTRL group (control): individuals who did not smoke marijuana or tobacco (n = 11); Group M: Marijuana smokers (n = 13); Group T: Tobacco smokers (n = 13); Group M + T: Smokers of both marijuana and tobacco (n = 08). Results: Smokers of both marijuana and tobacco led an increase of micronucleated cells on buccal mucosa when compared to control group. The occurrence of karyolysis showed significant changes in this group as well. The comet assay data revealed genetic damage in peripheral blood cells for all groups of smokers. Conclusion: In summary, our results showed that marijuana and /or tobacco are able to induce genetic damage and cytotoxicity in oral and peripheral blood cells.


Main Subjects

Volume 21, Issue 5
May 2020
Pages 1235-1239
  • Receive Date: 26 February 2019
  • Revise Date: 25 July 2019
  • Accept Date: 10 November 2019
  • First Publish Date: 01 May 2020