Cross-Protective IgG and IgA Antibodies against Oncogenic and Non-Oncogenic HPV Genotypes

Costa, Ana Paula; Giraldo, Paulo César; Cobucci, Ricardo Ney; Consolaro, Márcia Lopes; Souza, Raquel Pantarotto; Canário, Luanda Barbara; Machado, Paula Renata; Randall Martins, Rand; Vieira Baptista, Pedro; Jr, José Eleutério; Gonçalves, Ana Katherine

doi:10.31557/APJCP.2020.21.9.2799

Cross-Protective IgG and IgA Antibodies against Oncogenic and Non-Oncogenic HPV Genotypes

Document Type : Research Articles

Authors

¹ Postgraduate Program in Health Sciences, Federal University of Rio Grande do Norte, Natal, Brazil.

² Department of Gynecology, and Obstetrics, State University of Campinas, Campinas, Brazil.

³ Postgraduate Program in Biotechnology, Potiguar University, Natal, Brazil.

⁴ Clinical Cytology and STD Laboratory, Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, PR, Brazil.

⁵ Department of Clinical Analysis and Toxicology, Federal University of Rio Grande do Norte, Natal, Brazil.

⁶ Department of Pharmacy, Federal University of Rio Grande do Norte, Natal, Brazil.

⁷ Hospital Lusíadas Porto and Unidade de Tracto Genital Inferior, Serviço de Ginecologia e Obstetrícia, Centro Hospitalar de São João, Porto, Portugal.

⁸ Department of Female, Child and Adolescent Health, Federal University of Ceará, Fortaleza, Brazil.

10.31557/APJCP.2020.21.9.2799

Abstract

Objective: The aim of the study was to describe the course of IgG/IgA immune response in women immunized with bivalent vaccine and in women non-vaccinated with HPV infection, as well as evaluating the cross-protection against non-vaccine HPV types. Methods: Serum and cervical mucus samples were collected from infected and vaccinated women for HPV detection/genotyping and for detection of IgG/IgA anti-HPV/VLP (Virus-like Particles) by ELISA. Results: The median absorbance detected in serum samples for anti-HPV-IgG antibodies was higher in vaccinated women when compared to HPV infected women (p <0.01), however, the median absorbance in cervical mucus samples for anti-HPV-IgA was higher in infected women when compared to vaccinated women (p <0.01). Additionally, our analyses also provided additional evidence for cross-protective efficacy of the HPV-16/18 vaccine against HPV-82, -6, -11, -13, -61, -72 and -74. Conclusion: The IgG antibodies were significantly more detected in the serum of vaccinated women, while the IgA was found in greater quantities in cervical samples from those infected by the virus. In addition, there is evidence that the bivalent vaccine provides cross-protection against other non-oncogenic viral subtypes.

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