EZB-ICR Cell Line: A New Established and Characterized Oral Squamous Cell Carcinoma Cell Line From Tongue

Document Type : Research Articles


1 Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

2 Research Center of Otolaryngology Head and Neck Surgery, Shiraz University of Medical Sciences, Shiraz, Iran.

3 Department of Otolaryngology, School of Medicine, Shiraz University of Medical Sciences,Shiraz, Iran.


Background: Tongue cancer is one of the most aggressive forms of oral squamous cell carcinoma which needs more investigations. Herein, we aimed to establish and characterize a tongue cancer cell line. Methods: Tumor tissue was obtained from a 70-year-old woman with tongue cancer. The established cell line named as EZB-ICR and characterized for doubling time, expression of specific markers, HPV corporation and migration status using flow cytometry, immunofluorescence staining, multiplex PCR, and migration assay. Results: EZB-ICR was negative for expression of mesenchymal specific markers, cytokeratin19, pan-cytokeratin, vimentin and EPCAM, but was positive for S100 and Nestin. No appearance of human papilloma virus DNA was seen. The doubling time of EZB-ICR was 31 hours and migration assay confirmed its metastatic nature. Conclusion: To the best of our knowledge, EZB-ICR is the first tongue human cancer cell line established in Iran, and its features make it appropriate for cancer-based in vitro studies and contribute to more studies on tongue cancer.


Main Subjects

Volume 22, Issue 1
January 2021
Pages 99-103
  • Receive Date: 29 July 2020
  • Revise Date: 15 October 2020
  • Accept Date: 08 January 2021
  • First Publish Date: 08 January 2021