Profiling of Bile Microbiome Identifies District Microbial Population between Choledocholithiasis and Cholangiocarcinoma Patients

Document Type : Research Articles

Authors

1 Biomedical Sciences Program, Graduate School, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.

2 Cholangiocarcinoma Research Institute, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.

3 Department of Parasitology, Faculty of Medicine, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.

4 Department of Radiology, Faculty of Medicine, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.

5 Center for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.

6 Department of Pathology, Faculty of Medicine, Khon Kaen University, Nai Mueang, Khon Kaen, Thailand.

Abstract

Objective: Choledocholithiasis (CDL), a potential risk for cholangiocarcinoma (CCA) development, is often a consequence of bacterial infection. Thus, the microbial population that contributes to CDL might also be involved in CCA development. We compared the microbiome in bile fluid of CDL patients and CCA patients. Methods: Bile samples were collected from CDL (n = 30) and CCA (n =30) patients. Microbial profiling was performed individually by the sequencing of V3-V4 regions of the 16S rRNA gene. Results: Enterobacter, Pseudomonas, and Stenotrophomonas species were much more abundant in bile samples from CCA compared to CDL (p

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