Anti-Glioma Effect of Pseudosynanceia melanostigma Venom on Isolated Mitochondria from Glioblastoma Cells

Document Type : Research Articles


Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.


Background: Glioblastoma is the most common primary malignant tumor of the central nervous system that
occurs in the spinal cord or brain. Pseudosynanceia melanostigma is a venomous stonefish in the Persian Gulf, which
our knowledge about is little. This study’s goal is to investigate the toxicity of stonefish crude venom on mitochondria
isolated from U87 cells. Methods: In the first stage, we extracted venom stonefish and then isolated mitochondria have
exposed to different concentrations of venom. Finally, mitochondrial toxicity parameters (Succinate dehydrogenase
(SDH) activity, Reactive oxygen species (ROS), cytochrome c release, Mitochondrial Membrane Potential (MMP),
and mitochondrial swelling) have evaluated. Results: To determine mitochondrial parameters, we used 115, 230, and
460 μg/ml concentrations. The results of our study show that the venom of stonefish selectively increases upstream
parameters of apoptosis such as mitochondrial swelling, cytochrome c release, MMP collapse and ROS. Conclusion:
This study suggests that Pseudosynanceia melanostigma crude venom has selectively caused toxicity by increasing
active mitochondrial oxygen radicals. This venom could potentially be a candidate for the treatment of glioblastoma.


Main Subjects