Association between HOTAIR Polymorphisms and Lymphoma

Kashani, Sara; Sasan, Hosein Ali; Bahari, Gholamreza; Mollashahi, Behrouz; Hashemi, Seyed Mehdi; Taheri, Mohsen

doi:10.31557/APJCP.2021.22.9.2831

Association between HOTAIR Polymorphisms and Lymphoma

Document Type : Research Articles

Authors

¹ Department of Biology, Faculty of Science, Shahid Bahonar University, Kerman, Iran.

² Children and Adolescent Health Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran.

³ Genetics of Non-communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.

⁴ Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.

⁵ Department of Genetic, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.

10.31557/APJCP.2021.22.9.2831

Abstract

Introduction: The long non-coding RNA, HOTAIR, involved in cancer initiation and development. Objective: The aim of present study was to investigate the association of single nucleotide polymorphisms in the HOTAIR gene with lymphoma. Methods: We conducted a case-control study of 156 individuals with non-Hodgkin Lymphoma (NHL), 53 individuals with Hodgkin’s lymphoma (HL), and 245 unrelated healthy individuals to identify the genotype frequencies of each polymorphism. Genotyping of the SNPs (rs920778 T>C, rs1899663 G>T, rs4759314 A>G and rs12826786 C>T) was carried out using the polymerase chain reaction-restriction fragment length polymorphism. Results and Conclusion: The finding showed that rs1899663 variant of HOTAIR gene significantly decreased the risk of NHL in codominant, dominant, over-dominant and allelic inheritance models. We did not find any association between HOTAIR rs12826786, rs920788 and rs4759314 variants and NHL. The results indicated that neither the overall chi-square comparison of the cases and controls, nor the logistic regression analysis showed any association between HOTAIR polymorphisms and HL. Conclusively, our findings showed that rs1899663 of HOTAIR significantly decreased the risk of non-Hodgkin Lymphoma.

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