Molecular Noninvasive Diagnosis of Hepatocellular Carcinoma Using Microsatellite Instability

Document Type : Research Articles


1 Departement of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Giza, Egypt.

2 Department of Pathology, Theodor Bilharz Research Institute, Egypt.

3 HBP Surgery, National Hepatology and Tropical Medicine Research Institute, Egypt.

4 Department of Surgery, Theodore Bilharz Research Institute, Giza, Egypt.

5 Department of Hepato-Pancreatic-Biliary Surgery, National Hepatology and Tropical Medicine Research Institute, Cairo, Egypt.


Objective: Hepatocellular carcinoma (HCC) accounts for more than 80% of primary liver cancers. Moreover, in the next 10 years, more than one million patients are expected to die from liver cancer as estimated by the World Health Organization. The aim of the present study is to define the microsatellite phenotype in the blood, tumor and nontumor tissue samples from hepatocellular carcinoma cases to develop a simple non-invasive method for diagnosis and detection of the disease. Methods: A total of 100 patients with histologically-proven HCC were enrolled in this study, blood samples and tissue specimens from tumor and nontumor tissue were obtained from each patient. DNA was extracted and microsatellite instability MSI status was determined by polymerase chain reaction (PCR) using 5 mononucleotide and 5 dinucleotide repeats. Results: Among the 100 HCC tumors analyzed, (8%) considered as displaying a typical MSI-H phenotype as defined by instability in at least 3 of the 10 repeats analyzed, (61%) tumors displayed MSI-L and (31%) displayed MSS while in plasma the instability was (40%) for MSI-H, (44%) for MSI-L and (16%) for MSS. Conclusion: our findings could point to the achievement that HCC patients could be diagnosed by MSI analysis using blood sample as non-invasive way and this conclusion achieved our aim as the study shows impressive and promising results.


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