Effects of Vitamin E on Doxorubicin Cytotoxicity in Human Breast Cancer Cells in Vitro

Document Type : Research Articles


1 Gastrointestinal Cancer Research Center, Non-Communicable Disease Institute, Mazandaran University of Medical Science, Sari, Iran.

2 Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.


Objective: This study aimed to evaluate in vitro synergistic anticancer effect of doxorubicin combined with Vitamin E. Methods: The MTT assay was utilized to assess the cytotoxicity of Vitamin E and vitamin E combined with doxorubicin and vital activities of SKBR3, MDA-MB-231, and HFF cells over a 24-hour incubation period. In addition, the antioxidant properties of these interventions and the decrease of reactive oxygen species (ROS) content caused by the treatment were evaluated. Results: The antiproliferative effect of doxorubicin increased significantly in combination with vitamin E (Doxcorobicin 2µM vs. Vitamin E 120µM, P=0.000). Despite reducing cell ROS content due to vitamin E treatment, the combination of vitamin E and doxorubicin showed no significant synergistic effect (Doxcorobicin 2µM vs. Vitamin E 120µM, P=0.998). Conclusion: This study indicated that the doxorubicin–vitamin E treatment reduced the viability of breast cancer cells with the minimum side effects on normal cells. In addition, the high dosage of vitamin E intensified the cytotoxicity of doxorubicin.


Main Subjects

Volume 23, Issue 1
January 2022
Pages 201-205
  • Receive Date: 16 August 2021
  • Revise Date: 02 November 2021
  • Accept Date: 08 January 2022
  • First Publish Date: 08 January 2022