Microscopic Evaluation of Ovarian Surface Epithelium Following Treatment with Conjugated Estrogens in a Mouse Model

Al-Amri, Issa S; Kadim, Isam T; Al-Kindi, Abdul Aziz; Khalaf, Samera K; Al-Harrasi, Ahmad S; Al-Hashmi, Sulaiman A; Al-Shibli, Ahood A; Al-Hadi, Wesal M; Al-Mahimuli, Manal K; Jamshidi-Adegani, Fatemeh; Vakilian, Saeid; Al-Amri, Asala I; Al-Yaqoobi, Sausan S; Al-Riyami, Khamis O

doi:10.31557/APJCP.2022.23.6.1913

Microscopic Evaluation of Ovarian Surface Epithelium Following Treatment with Conjugated Estrogens in a Mouse Model

Document Type : Research Articles

Authors

¹ Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, Birkat Al-Mouz, Nizwa, Sultanate of Oman.

² Natural and Medical Sciences Research Center, University of Nizwa, Birkat Al-Mouz, Nizwa, Sultanate of Oman.

³ DARIS Center for Scientific Research and Technology Development, University of Nizwa, Birkat Al-Mouz, Nizwa, Sultanate of Oman.

10.31557/APJCP.2022.23.6.1913

Abstract

Background: This study was designed to evaluate the effect of different concentrations of conjugated equine estrogens (CEE) on the ovarian epithelium of female CD1 mice. Methods: Twenty-four female mice at 7 months with irregular estrus cycles were randomly divided into four groups of 6 mice each. Group one was considered as a control group and received a daily dose of 0.5ml of propylene glycol, for three weeks, while those in the treatment groups received a daily dose of 14μg/kg, 28μg/kg and 56μg/kg conjugated equine estrogens, respectively. Results: The results from this study showed a strong correlation between elevated concentrations of CEE and histological changes in ovarian surface epithelium (OSE). They also showed that administration of high-dose estrogen created the conditions for excessive proliferation of OSE which may progress into the development of cysts in the ovaries. Conclusion: This study concluded that high concentrations of CEE may increase the chances of developing epithelial ovarian cancer.

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