Document Type : Research Articles
Authors
1
Department of Chemical Pathology, Medical Research Institute, Alexandria University, Egypt.
2
Department of Pharmacology and Therapeutics, Faculty of Pharmacy, Pharos University, Egypt.
3
Lecturer in the School of Biological Sciences, Faculty of Science, University of East Anglia, UK.
4
Department of Pathology, Medical Research Institute, Alexandria University, Egypt.
5
Department of Surgery, Medical Research Institute, Alexandria University, Egypt.
6
Department of Cancer Management and Research, Medical Research Institute, Alexandria University, Alexandria, Egypt.
7
Centre hospitalier de Troyes, radiotherapy department, France. 8MB BCh , October 6 University, Egypt.
8
MB BCh , October 6 University, Egypt.
Abstract
Objective: evaluating the role of FOXO3 mRNA and mi RNA 182-5P expression levels in BC patients. Method: 25 Samples of breast cancer and paired samples of non-cancerous tissues from the same resected breast were obtained from 25 female patients suffering from breast cancer and examined and analyzed by real time PCR to detect the expression levels of FOXO3 mRNA and mi RNA 182-5P. Patients’ data were collected from patients medical records. Results: Foxo3 m RNA expression was down regulated in BC tissues (1.37± 1.96) as compared to control group (23.62 ± 54.39) and decreased FOXO3 expression was associated with larger tumor size (p= 0.046), late histopathological grading (p= 0.002), late TNM staging (<0.001) and increased miR-182 expression (p= 0.025). We found that expression level of miR-182 was significantly higher among breast cancer group (1.10±1.15) as compared to the control group (0.58±0.96 ) with p value = 0.017. We noted a significant increased expression associated with larger tumor size (p= 0.002), late histopathological grading (p= 0.008), late TNM staging (p= 0.002) and decreased FOXO3 expression (p= 0.025). A significant negative correlation between miR-182 and FOXO3 mRNA fold expression with r = - 0.447, and a p value of 0.025, this could be attributed to miRNA targeting FOXO gene. Coclusion: Down regulation of FOXO3 and up regulation of miR-182 expression was associated with advanced breast cancer. The negative correlation between miR-182 and FOXO3 mRNA could be attributed to miRNA targeting FOXO gene.
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