Single Nucleotide Polymorphisms of ADH1B, ADH1C and ALDH2 Genes in 235 People Living in Thai Nguyen Province of Vietnam

Hoang, Yen Thi Thu; Nguyen, Yen Thi; Nguyen, Hai Dinh; Le, Anh Thi Phuong; Bui, Huong Thi Thu; Vu, Nhung Phuong; Nguyen, Ha Hai

doi:10.31557/APJCP.2022.23.12.4243

Single Nucleotide Polymorphisms of ADH1B, ADH1C and ALDH2 Genes in 235 People Living in Thai Nguyen Province of Vietnam

Document Type : Research Articles

Authors

¹ Department of Genetics and Molecular Biology, Faculty of Biotechnology, Thai Nguyen University of Sciences, Thai Nguyen University, Thai Nguyen province, Vietnam.

² Department of Biochemistry, Faculty of Foundation Medicine, Thai Nguyen University of Medicine and Pharmacy, Thai Nguyen University, Thai Nguyen province,Vietnam.

³ Genome Analysis Laboratory, Institute of Genome Research, Vietnam Academy of Science and Technology, Hanoi, Vietnam.

10.31557/APJCP.2022.23.12.4243

Abstract

Objective: ADH1B, ADH1C and ALDH2 genes are mainly responsible for alcohol metabolism in the body. Several
single nucleotide polymorphisms (SNPs) of these genes have been reported to be associated with alcohol dependence
and are considered risk factors for various human diseases. This study aims to identify the prevalence of three SNPs of
ADH1B (rs1229984), ADH1C (rs698) and ALDH2 (rs671) in 235 unrelated individuals living in Thai Nguyen province,
the northeast region of Vietnam. Methods: The target genotypes were identified by using PCR direct sequencing, and
their frequencies were compared to previous reports. Result: Our data showed that allele frequencies of ADH1B*2
(rs1229984), ADH1C*2 C (rs698) and ALDH2*2 (rs671) were 68.8%, 8.3% and 20.4%, respectively. The ADH1B*2
and ADH1C*2 frequencies were similar to those of the Kinh ethnic individuals living in the south region of Vietnam,
while the ALDH2*2 frequency was higher. Compared to data from other countries, ADH1B*2 frequency is similar to
the Philippines (60.5%) and Mongolia (62.9%) but significantly different from the other populations. The ADH1C*2
frequency is not so different compared to Japanese (5.7%) and Chinese (7.1%) but is quite different in other populations.
ALDH2*2 frequency was lower than Japanese (29.3%), Indonesian (30%) and higher than other countries. Regarding
the risk of alcoholism, the percentage of Vietnamese people in this study with genotypes related to alcohol dependence
is 8.1%. In contrast, the carrier has genotypes protecting against alcoholism with high frequency, 91.9%. Among them,
the individuals can cause high acetaldehyde accumulation accounting for 33.2%. Conclusion: This study helps to
understand the genetic polymorphisms of alcohol metabolism genes in the community living in Thai Nguyen province,
northeast of Vietnam, and provides valuable scientific data relating to alcohol consumption behavior as well as public
health protection.

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